Structure- and Design-Based Difficulties in Recombinant Protein Purification in Bacterial Expression

Advances and challenges in recombinant protein expression in Escherichia coli

Recombinant protein expression and purification

Recent advances in genomics, proteomics, and bioinformatics have facilitated the use of recombinant DNA technology in order to evaluate any protein of interest, without prior knowledge of the protein’s cellular location or function. The parallel use of affinity tags with recombinant DNA techniques, allows the facile modification of proteins of interest leading to efficient identification, produ...

Prokaryotic Expression, Purification, and Polyclonal Antibody Production of a Truncated Recombinant Rabies Virus L Protein

Background: Rabies virus (RABV) is a deadly neurotropic virus that causes the disease of rabies in humans and animals. L protein is one of the large structural protein of rabies virus, which displays multiple enzymatic activities, and is required for viral transcription and replication. Objectives: A truncated L protein of Rabies virus is being cloned, expressed and purified to produce relevant...

Expression and Purification of Recombinant Outer Surface Protein D of Borrelia burgdorferi

To carry out the immunological experiments on the serum of Multiple Sclerosis (MS) patients, based on a correlation between Borrelia burgdorferi infection and contracting MS autoimmune disease the outer surface protein D (OspD) of the bacterium was expressed and purified. A clone containing the OspD gene in pET11a expression vector under the control of T7 promoter was transformed to the bacteri...

Gene Expression Systems and Recombinant Protein Purification

There is no substance as important as deoxyribonucleic acid as it carries within its own structure the hereditary in formations that determines the structure of proteins, the important molecule of life. Deoxyribonucleic acid transcribe messenger ribonucleic acid which finally translates protein the essential macromolecules. Through gene expression the information contained in gene can be utiliz...